Difference between revisions of "Part:BBa K2719012"
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<partinfo>BBa_K2719012 short</partinfo> | <partinfo>BBa_K2719012 short</partinfo> | ||
− | + | This part facilitates the selection of transformed colonies for the production of Tenascin (BBa_K2719005) by the incorporation of a reporter cassette (BBa_K592015), which constitutively expresses blue chrome protein. This reporter simplifies the selection of colonies by the appearance of blue colored colonies, serving as transformation and ligation control. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 13:12, 17 October 2018
TCD5 coding device with blue chromoprotein
This part facilitates the selection of transformed colonies for the production of Tenascin (BBa_K2719005) by the incorporation of a reporter cassette (BBa_K592015), which constitutively expresses blue chrome protein. This reporter simplifies the selection of colonies by the appearance of blue colored colonies, serving as transformation and ligation control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1257