Difference between revisions of "Part:BBa K2633006:Experience"

 
 
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===Applications of BBa_K2633006===
 
===Applications of BBa_K2633006===
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This part was transformed using a restriction site illegal version of BBa_K2633000[https://parts.igem.org/Part:BBa_K2633000]. It was then used to inoculate ''Brachypodium distachyon'' seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation.
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Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate.
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https://static.igem.org/mediawiki/parts/d/db/T--MichiganState--transformation_results_small.png
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Figure 2.[https://static.igem.org/mediawiki/parts/thumb/1/13/T--MichiganState--root_colonization_3wk_GA_results_full.png/900px-T--MichiganState--root_colonization_3wk_GA_results_full.png] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies about 2 mm long in ''B. distachyon'' roots
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B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.
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https://static.igem.org/mediawiki/parts/6/66/T--MichiganState--root_colonization_3wk_GA_results_small.png
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Figure 3.[https://static.igem.org/mediawiki/parts/thumb/c/c4/T--MichiganState--root_colonization_4wk_GA_results_full.png/900px-T--MichiganState--root_colonization_4wk_GA_results_full.png] 4 weeks post inoculation on plant roots. A) FCP2-01 on ''B. distachyon'' roots. B) FCP2-0 colonizing root hairs.
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C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.
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https://static.igem.org/mediawiki/parts/f/ff/T--MichiganState--root_colonization_4wk_GA_results_small.png
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 01:49, 16 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2633006

This part was transformed using a restriction site illegal version of BBa_K2633000[1]. It was then used to inoculate Brachypodium distachyon seeds. Transformed bacteria expressing GFP were observed on and inside roots from 1 to 4 weeks post inoculation.

Figure 1. A) FCP2-01 transformed with GFP (left) vs wild-type (right) under blue light with orange filter. B) Transformed colonies on an agar plate.

T--MichiganState--transformation_results_small.png

Figure 2.[2] 3 weeks post inoculation on plant roots. A) FCP2-01 forming large colonies about 2 mm long in B. distachyon roots B) Detail of panel A. C) FCP2-01 growing between plant cells on the root surface. D) Detail of colonies on root epidermis.

T--MichiganState--root_colonization_3wk_GA_results_small.png

Figure 3.[3] 4 weeks post inoculation on plant roots. A) FCP2-01 on B. distachyon roots. B) FCP2-0 colonizing root hairs. C) FCP2-01 growing on epidermis of the root. D) Enlarged image of panel C.

T--MichiganState--root_colonization_4wk_GA_results_small.png

User Reviews

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