Difference between revisions of "Part:BBa K2633006"

Latest revision as of 02:50, 18 October 2018

Enterobacter ludwigii FCP2-01

This organism is a transformable gram-negative bacterium which was isolated from surface-sterilized switchgrass (Panicum virgatum) roots. It is capable of reinfecting roots of Brachypodium distachyon. Enterobacter ludwigii can be transformed using [http://2018.igem.org/Team:MichiganState/Experiments electroporation]. It is susceptible to chloramphenicol and streptinomycin. Contact Julian Liber (liberjul@msu.edu) if a sample of this strain is desired. The protocols developed to make electrocompetent cells and conduct transformations for this chassis are available [http://2018.igem.org/Team:MichiganState/Experiments here].

MichiganState 2018 transformed this chassis using plasmids with thepSB1C3 backbone, this first documented use of this plasmid in an Enterobacter species. The part we designed for expression of an acdS-eGFP fusion is BBa_K2633000, which allows for fluorescent microscopy of the bacteria in situ and expression of the plant growth promoting enzyme ACC deaminase.

The 16S rRNA gene sequence can be found here. The Hsp60 sequence can be found here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2633006 short</partinfo>
-This organism is a transformable gram-negative bacterium which was isolated from surface-sterilized switchgrass (''Panicum virgatum'') roots. It is capable of reinfecting roots of ''Brachypodium distachyon''.
+This organism is a transformable gram-negative bacterium which was isolated from surface-sterilized switchgrass (''Panicum virgatum'') roots. It is capable of reinfecting roots of ''Brachypodium distachyon''. ''Enterobacter ludwigii'' can be transformed using [http://2018.igem.org/Team:MichiganState/Experiments electroporation]. It is susceptible to chloramphenicol and streptinomycin. Contact Julian Liber (liberjul@msu.edu) if a sample of this strain is desired. The protocols developed to make electrocompetent cells and conduct transformations for this chassis are available [http://2018.igem.org/Team:MichiganState/Experiments here].
+MichiganState 2018 transformed this chassis using plasmids with the[https://parts.igem.org/Part:pSB1C3 pSB1C3 backbone], this first documented use of this plasmid in an ''Enterobacter'' species. The part we designed for expression of an ''acdS''-eGFP fusion is [https://parts.igem.org/Part:BBa_K2633000 BBa_K2633000], which allows for fluorescent microscopy of the bacteria ''in situ'' and expression of the plant growth promoting enzyme ACC deaminase.
+The 16S rRNA gene sequence can be found [https://static.igem.org/mediawiki/parts/5/51/T--MichiganState--FCP2-01_16S_rRNA_gene.txt here]. The Hsp60 sequence can be found [https://static.igem.org/mediawiki/2018/3/35/T--MichiganState--FCP2-01_Hsp60.txt here].
 <!-- Add more about the biology of this part here