Difference between revisions of "Part:BBa K2622035"

 
 
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<partinfo>BBa_K2622035 short</partinfo>
 
<partinfo>BBa_K2622035 short</partinfo>
  
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scFv_antiVGY-IgA-Mstx long description
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ScFv_antiVGY-IgA-Mstx has a ScFv_antiVGY (BBa_K2622004) fused to translocator domain's (BBa_K2622003) N-terminus, so that it could be displayed and detected in the outer surface of the cholesterol-free membrane by erythrocyte lysis assay. On the C-terminus it has a Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.
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This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI and SpeI sites that must be avoided when cloning.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:39, 17 October 2018


scFv_antiVGY-IgA-Mstx

ScFv_antiVGY-IgA-Mstx has a ScFv_antiVGY (BBa_K2622004) fused to translocator domain's (BBa_K2622003) N-terminus, so that it could be displayed and detected in the outer surface of the cholesterol-free membrane by erythrocyte lysis assay. On the C-terminus it has a Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI and SpeI sites that must be avoided when cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
    Illegal SpeI site found at 813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 813
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
    Illegal SpeI site found at 813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal SpeI site found at 813
    Illegal NgoMIV site found at 1716
    Illegal AgeI site found at 1491
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI.rc site found at 20