Difference between revisions of "Part:BBa K2557002"

Latest revision as of 18:28, 17 October 2018

Bxb1 attB-RFP-Bxb1 attP

We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.

Usage and Biology

Sequence and Features

Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T undergone different experimental treatments. Transfection of different amounts of plasmids containing recombinase genes into cells.

Fig. 1 shows that this composite part works fine in the HEK 293T.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 837
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 36
Illegal BsaI site found at 177
Illegal BsaI.rc site found at 885
Illegal SapI site found at 794

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
+=== Sequence and Features===
+<html>
+<img src="https://static.igem.org/mediawiki/parts/2/28/T--NAU-China--attB-attP.png"width="300"/>
+</html>
+<html>
+<img src="https://static.igem.org/mediawiki/parts/4/47/T--NAU-China--AttL-attR.png"width="300"/>
+</html>
+===Characterization===
+<html>
+<img src="https://static.igem.org/mediawiki/parts/e/e9/T--NAU-CHINA-hongse.jpg"width="600"/>
+</html>
+Fig. 1 Fluorescence microscope observation of HEK 293T  undergone different experimental treatments. Transfection of different amounts of plasmids containing recombinase genes into cells.
+Fig. 1 shows that this composite part works fine in the HEK 293T.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2557002 SequenceAndFeatures</partinfo>
 ===Functional Parameters===
 <partinfo>BBa_K2557002 parameters</partinfo>