Difference between revisions of "Part:BBa K2541402:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from .So it is BbsI restriction site free and can be used in GoldenGate assembly.
+
sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from .So it is BbsI restriction site free and can be used in Golden Gate assembly.
  
 
===Source===
 
===Source===
−
The sfGFP (K2541400) is BbsI restriction site free and can be used in GoldenGate assembly. We get this sequence from NCBI. And other sequences are derived from Registry of Standard Biological Parts.
+
The sfGFP (K2541400) is BbsI restriction site free and can be used in Golden Gate assembly. We get this sequence from NCBI. And other sequences are derived from Registry of Standard Biological Parts.
  
 
===References===
 
===References===

Latest revision as of 14:32, 15 October 2018


sfGFP_optimism measurement device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sfGFP(BbsI free) has two mutations from superfolder GFP(BBa_I746916)- from .So it is BbsI restriction site free and can be used in Golden Gate assembly.

Source

The sfGFP (K2541400) is BbsI restriction site free and can be used in Golden Gate assembly. We get this sequence from NCBI. And other sequences are derived from Registry of Standard Biological Parts.

References

[1]Southward CM, Surette MG. 2002. The dynamic microbe: green fluorescent protein brings bacteria to light. Mol. Microbiol. 45:1191–1196.

[2]Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38.

[3]Pédelacq J-D, Cabantous S, Tran T, Terwilliger TC, Waldo GS. 2006. Engineering and characterization of a superfolder green fluorescent protein. Nat. Biotechnol. 24:79 –88.

[4]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490.

[5]Segall-Shapiro T H, Sontag E D, Voigt C A. Engineered promoters enable constant gene expression at any copy number in bacteria[J]. Nature Biotechnology, 2018.