Difference between revisions of "Part:BBa K2610030"

 
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<partinfo>BBa_K2610030 short</partinfo>
 
<partinfo>BBa_K2610030 short</partinfo>
  
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Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes
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Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.
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===Usage and Biology===
 
===Usage and Biology===
  
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry.
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, we have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry.
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We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.   
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We fused this promotor to green fluorescent protein (see part [https://parts.igem.org/Part:BBa_K2610031 BBa_K2610031]) and stressed transformed DH5a cells for several hours.   
  
 
[[File:T--Leiden--heatmap.png|750px]]  
 
[[File:T--Leiden--heatmap.png|750px]]  
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<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>  
 
<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>  
  
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As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide.
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As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide, which both cause oxidative stress.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:30, 17 October 2018


pSoxS

Regulatory protein SoxS is involved in the oxidative stress signaling pathway in Escherichia coli. Intracellular superoxide-generating compounds cause the SoxR to activate transcription of SoxS, which then triggers a set of defense and repair genes that form the oxidative response system.


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. For this, we have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused this promotor to green fluorescent protein (see part BBa_K2610031) and stressed transformed DH5a cells for several hours.

T--Leiden--heatmap.png

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the SoxS promoter is specifically activated by nalidixic acid and hydrogen peroxide, which both cause oxidative stress.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]