Difference between revisions of "Part:BBa K2805006"
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<partinfo>BBa_K2805006 short</partinfo> | <partinfo>BBa_K2805006 short</partinfo> | ||
| − | + | This part codes for the helix-loop-helix protein PIF3 linked to an NLS and the VP16 transcriptional activator. This is piece of the PhiReX system which dimerizes with PhyBNT (BBa_K2805005) when PhyBNT is induced by red (650 nm wavelength) light. This PIF3 is fused to a transcriptional activator, so it will activate transcription of a downstream gene under the influence of red light and in the presence of PhyBNT-Zif268. | |
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Latest revision as of 04:20, 17 October 2018
PIF3-NLS-VP16
This part codes for the helix-loop-helix protein PIF3 linked to an NLS and the VP16 transcriptional activator. This is piece of the PhiReX system which dimerizes with PhyBNT (BBa_K2805005) when PhyBNT is induced by red (650 nm wavelength) light. This PIF3 is fused to a transcriptional activator, so it will activate transcription of a downstream gene under the influence of red light and in the presence of PhyBNT-Zif268.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1065
Illegal SpeI site found at 1461
Illegal PstI site found at 1921 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1461
Illegal PstI site found at 1921 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 885
Illegal BglII site found at 1400
Illegal BamHI site found at 2484
Illegal XhoI site found at 2727 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1065
Illegal SpeI site found at 1461
Illegal PstI site found at 1921 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1065
Illegal SpeI site found at 1461
Illegal PstI site found at 1921 - 1000COMPATIBLE WITH RFC[1000]