Difference between revisions of "Part:BBa K2570007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | ||
| + | Primers: | ||
| + | |||
| + | Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’ | ||
| + | |||
| + | Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’ | ||
| + | |||
| + | |||
===Source=== | ===Source=== | ||
| − | Organism="Escherichia coli str. K-12 substr. MG1655". EC_number="2.8.3.9". Protein_id="AAC75281.1". | + | Organism="''Escherichia coli str. K-12 substr. MG1655''". EC_number="2.8.3.9". Protein_id="AAC75281.1". |
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759 | https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759 | ||
===References=== | ===References=== | ||
Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76. | Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76. | ||
Latest revision as of 08:46, 16 October 2018
atoA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 180
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers:
Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’
Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’
Source
Organism="Escherichia coli str. K-12 substr. MG1655". EC_number="2.8.3.9". Protein_id="AAC75281.1". https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759
References
Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.