Difference between revisions of "Part:BBa K2717022:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | NOTHING | |
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===Source=== | ===Source=== | ||
| − | + | Sequences were attained from E. coli F12K using PCR. We added lacO and rbs in the 5’ of mpra while v5 and 6*his tag in the 3’ of it. Besides, we also add emrr Binding Promoter and rbs in the 5’ of mcherry. After that we fused the two altered sequences and gain this plasmid. | |
===References=== | ===References=== | ||
Latest revision as of 18:11, 17 October 2018
jqy-hairpin(mprA-Protection coding3-mCherry reverse-scar4-emrr reverse)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 451
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 776
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 556
Design Notes
NOTHING
Source
Sequences were attained from E. coli F12K using PCR. We added lacO and rbs in the 5’ of mpra while v5 and 6*his tag in the 3’ of it. Besides, we also add emrr Binding Promoter and rbs in the 5’ of mcherry. After that we fused the two altered sequences and gain this plasmid.