Difference between revisions of "Part:BBa K2610009"
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<partinfo>BBa_K2610009 short</partinfo> | <partinfo>BBa_K2610009 short</partinfo> | ||
| − | Protein RecA allows for homologous recombination and is essential in the repair and maintenance of E.coli DNA. It has been shown that RecA is upregulated in E.coli after administration of known carcinogenic agent MNNG. | + | Protein RecA allows for homologous recombination and is essential in the repair and maintenance of E.coli DNA. It has been shown that RecA is upregulated in E.coli after administration of known carcinogenic agent MNNG. <span class="plainlinks">[https://aem.asm.org/content/71/5/2338 (Norman et al, 2004)]</span> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 22:04, 15 October 2018
pRecA
Protein RecA allows for homologous recombination and is essential in the repair and maintenance of E.coli DNA. It has been shown that RecA is upregulated in E.coli after administration of known carcinogenic agent MNNG. (Norman et al, 2004)
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the RecA promoter is severely downregulated by multiple antibiotics.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]