Difference between revisions of "Part:BBa K2610007"
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| − | MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. | + | MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/11601842 (Delihas et al, 2001)]</span> |
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| + | ===Usage and Biology=== | ||
| + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours. </p> <br> | ||
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| + | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
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| + | As can be observed in Figure 1 the MicFpromoter is only moderately activated by tetracycline | ||
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Latest revision as of 22:01, 15 October 2018
pMicF
MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. (Delihas et al, 2001)
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the MicFpromoter is only moderately activated by tetracycline
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]