Difference between revisions of "Part:BBa K2717015:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | In the course of the experiment, GFP, T7 RNA Polymerase and gdh are obtained by PCR, then GFP is ligated with T7 RNA Polymerase by overlap method, and then our modified plasmid pUCyder is linearized by single restriction enzyme, through infusion. The way to connect the pieces together. | ||
===Source=== | ===Source=== | ||
| − | T7 | + | GFP is derived from the plasmid, T7 RNA Polymerase gene is derived from Escherichia coli BL21 (DE3), and gdh is derived from the genome of Escherichia coli K12. |
===References=== | ===References=== | ||
Latest revision as of 19:02, 17 October 2018
GFP-TGATG-T7 RNA Polymerase-GDH-v5 tag-his tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3389
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 5252
Illegal AgeI site found at 6144 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6126
Illegal BsaI.rc site found at 644
Design Notes
In the course of the experiment, GFP, T7 RNA Polymerase and gdh are obtained by PCR, then GFP is ligated with T7 RNA Polymerase by overlap method, and then our modified plasmid pUCyder is linearized by single restriction enzyme, through infusion. The way to connect the pieces together.
Source
GFP is derived from the plasmid, T7 RNA Polymerase gene is derived from Escherichia coli BL21 (DE3), and gdh is derived from the genome of Escherichia coli K12.