Difference between revisions of "Part:BBa K2533046"
 
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<partinfo>BBa_K2533046 short</partinfo>
 
<partinfo>BBa_K2533046 short</partinfo>
  
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transport of lactic acid
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transport lactate
  
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<!-- Add more about the biology of this part here
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<h1>'''Usage and biology'''</h1>
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===Usage and Biology===
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lldP refers to L-lactate permease, helping transport lactate out of the cell. With this gene, Rhodopseudomonas palustris could transport lactate out of the cell more efficiently, which brings Shewanella more carbon source.
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<h1>'''Characterization'''</h1>
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This is one section for lactate transportion part.
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[[File:T--HUST-China--2018-tonglu-lldp.png ‎|400px|thumb|center|Figure1. RBS-lldP]]
  
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<h2>DNA Gel Electrophoretic</h2>
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<span class='h3bb'>Sequence and Features</span>
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To make sure that we get the target gene, we did the DNA gel electrophoretic for verification. And here is the result.
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<partinfo>BBa_K2533046 SequenceAndFeatures</partinfo>
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[[File:T--HUST-China--2018-jiaotu-ldha and lldp.png|400px|thumb|center|Figure2. Verification of successful transformation of pSB1C3-RBS-lldP]]
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Our target gene is 1674bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
  
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<h2>Electrogenesis</h2>
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By detecting the production of lactate after expressing, we might find out whether lldP could effectively help Rhodopseudomonas palustris transport lactate out of cell.
 +
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3. shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.]]
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It could be demonstrated that the target gene could be expressed in the engineered cells. More lactate has been transported out of the cell by engineered bacteria, which helps to provide more carbon source for Shewanella.
  
 
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<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2533046 parameters</partinfo>
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<partinfo>BBa_K2533030 parameters</partinfo>
 
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Latest revision as of 23:04, 17 October 2018


RBS-lldP

transport lactate

Usage and biology

lldP refers to L-lactate permease, helping transport lactate out of the cell. With this gene, Rhodopseudomonas palustris could transport lactate out of the cell more efficiently, which brings Shewanella more carbon source.

Characterization

This is one section for lactate transportion part.

Figure1. RBS-lldP

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic for verification. And here is the result.

Figure2. Verification of successful transformation of pSB1C3-RBS-lldP

Our target gene is 1674bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By detecting the production of lactate after expressing, we might find out whether lldP could effectively help Rhodopseudomonas palustris transport lactate out of cell.

Figure3. shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieves the goal to help strains produce lactate.

It could be demonstrated that the target gene could be expressed in the engineered cells. More lactate has been transported out of the cell by engineered bacteria, which helps to provide more carbon source for Shewanella.