Difference between revisions of "Part:BBa K2717012"

Latest revision as of 20:14, 17 October 2018

RBS2000-GFP-hairpin-GDH-v5 tag-his tag

In this composite part, we link GFP and glucose dehydrogenase with hairpin structure and use Promoter lacI and RBS 2000 control the whole coding. The hairpin structure contains a 6x His tag, a stop codon and RBS, which make the transcription ratio of the downstream gene and the upstream gene is about 1:0.2. We designed this part to verify the positive feedback regulation mechanism of GDH.

function:

We designed this part to verify the positive feedback regulation mechanism of GDH: when gdh is expressed, the strain gains a growth advantage. At the same time, due to the hairpin structure, the expression level of GFP has been very high. gdh promotes the metabolism of strains, which means it will express more gdh to gain growth advantage, resulting in more GFP being expressed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2336
Illegal AgeI site found at 3228
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3210
Illegal BsaI.rc site found at 678

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2717012 short</partinfo>
-rbs2000-GFP-hairpin-gdh-tag
+In this composite part, we link GFP and glucose dehydrogenase with hairpin structure and use Promoter lacI and RBS 2000 control the whole coding. The hairpin structure contains a 6x His tag, a stop codon and RBS, which make the transcription ratio of the downstream gene and the upstream gene is about 1:0.2. We designed this part to verify the positive feedback regulation mechanism of GDH.
+function:
+We designed this part to verify the positive feedback regulation mechanism of GDH: when gdh is expressed, the strain gains a growth advantage. At the same time, due to the hairpin structure, the expression level of GFP has been very high. gdh promotes the metabolism of strains, which means it will express more gdh to gain growth advantage, resulting in more GFP being expressed.
 <!-- Add more about the biology of this part here