Difference between revisions of "Part:BBa K2610003"
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<partinfo>BBa_K2610003 short</partinfo> | <partinfo>BBa_K2610003 short</partinfo> | ||
| − | Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. | + | Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25128614 (Rodrigues et al, 2014)]</span> <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25173932 (Shi et al, 2014)]</span> |
| − | ==Usage and Biology | + | ==Usage and Biology== |
<p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br> | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br> | ||
Latest revision as of 21:32, 15 October 2018
pIbpB
Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. (Rodrigues et al, 2014) (Shi et al, 2014)
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the IbpB promoter is only moderately activated by tetracycline
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]