Difference between revisions of "Part:BBa K2610003"

 
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2610003 short</partinfo>
 
<partinfo>BBa_K2610003 short</partinfo>
  
−
Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB.
+
Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25128614 (Rodrigues et al, 2014)]</span> <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25173932 (Shi et al, 2014)]</span>
  
 +
==Usage and Biology==
 +
<p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br>
  
−
<!-- Add more about the biology of this part here
+
[[File:T--Leiden--heatmap.png|750px]] <br>
−
===Usage and Biology===
+
<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>
  
 +
As can be observed in Figure 1 the IbpB promoter is only moderately activated by tetracycline
 +
 +
<!-- Add more about the biology of this part here
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 21:32, 15 October 2018


pIbpB

Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. (Rodrigues et al, 2014) (Shi et al, 2014)

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the IbpB promoter is only moderately activated by tetracycline

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]