Difference between revisions of "Part:BBa K2610002"
 
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<partinfo>BBa_K2610002 short</partinfo>
 
<partinfo>BBa_K2610002 short</partinfo>
  
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===Usage and Biology===
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/25128614 (Rodrigues et al, 2014)]</span> </p>
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. <br> iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br>
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[[http://2018.igem.org/File:T--Leiden--heatmap.png|thumb|Figure 1 caption]]
 
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<br><img src="https://static.igem.org/mediawiki/2018/9/91/T--Leiden--heatmap.png" max-width="30%">
 
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<b>Figure 1.</b> Heatmap with stress-activated promoters
 
  
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As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
 
  
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===Usage and Biology===
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<p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br>
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[[File:T--Leiden--heatmap.png|750px]] <br>
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<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>
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As can be observed in Figure 1 the IbpA promoter is only moderately activated by streptomycin and tetracycline.
  
 
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Latest revision as of 21:31, 15 October 2018


pIbpA

Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. (Rodrigues et al, 2014)


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the IbpA promoter is only moderately activated by streptomycin and tetracycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]