Difference between revisions of "Part:BBa K2557037"
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Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology. | Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology. | ||
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− | < | + | ===Characterization=== |
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+ | <img src="https://static.igem.org/mediawiki/parts/c/ca/T--NAU-CHINA-sanzhang_lvse.jpg"width="600"/> | ||
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+ | Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence. | ||
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+ | Fig. 1 shows that EGFP can be normally expressed in HEK 293T, and the fluorescence intensity depends on the amount of expression. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2557037 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2557037 SequenceAndFeatures</partinfo> |
Latest revision as of 18:07, 17 October 2018
EGFP
Enhanced Green Fluorescent Protein is a fluorescent protein and is a widely used reporter gene in synthetic biology.
Characterization
Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence.
Fig. 1 shows that EGFP can be normally expressed in HEK 293T, and the fluorescence intensity depends on the amount of expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 6
- 1000COMPATIBLE WITH RFC[1000]