Difference between revisions of "Part:BBa K2665012:Design"

 
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===Design Notes===
 
===Design Notes===
 
We added TDH3 promoter and CYC1 teminator in order to express the ORF, and added His-tag in case of Western Blotting.
 
We added TDH3 promoter and CYC1 teminator in order to express the ORF, and added His-tag in case of Western Blotting.
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The ORF sequence is refered from Reference[1].
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===Source===
 
===Source===
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The ORF of this part is derived from Zygosaccharomyces rouxii.
 
The ORF of this part is derived from Zygosaccharomyces rouxii.
  
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===References===
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===Reference===
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[1] Hou,Lihua Wang,Meng Wang,Cong Wang,Chunling Wang,Haiyong (2013) Analysis of salt-tolerance genes in zygosaccharomyces rouxii, <i>Applied Biochemistry and Biotechnoloogy</i> 1417-1425
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Latest revision as of 02:13, 9 October 2018


TDH3-ZrGPD1-6xHis-CYC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1923
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1129
    Illegal BsaI site found at 1889
    Illegal BsaI.rc site found at 1271


Design Notes

We added TDH3 promoter and CYC1 teminator in order to express the ORF, and added His-tag in case of Western Blotting. The ORF sequence is refered from Reference[1].

Source

The ORF of this part is derived from Zygosaccharomyces rouxii.

Reference

[1] Hou,Lihua Wang,Meng Wang,Cong Wang,Chunling Wang,Haiyong (2013) Analysis of salt-tolerance genes in zygosaccharomyces rouxii, Applied Biochemistry and Biotechnoloogy 1417-1425