Difference between revisions of "Part:BBa K2683001:Design"

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===Design Notes===
 
===Design Notes===
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Codon optimization was ideal for characterization and application by using E.coli.  
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This part is codon optimized for use in <i> E.coli </i>.
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===Source===
 
===Source===
  
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The original sequence is derived from the Arc protein found in mouse neural cells. The DNA was amplified from the mouse genome and made biobrick compatable by using primer extension PCR and site directed mutagenesis.
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The original sequence is derived from the Arc protein found in mouse neural cells. https://www.uniprot.org/uniprot/Q9WV31
  
 
===References===
 
===References===
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[1]Pastuzyn, E., Day, C., Keams, R., Kyrke-Smith, M., Taibi, A., McCormick, J., Yoder, N., Belnap, D., Erlendsson, S., Morado, D., Briggs, J., Feschotte, C., and Shepard, J. (2018) The Neuronal Gene Arc encodes a repurposed retrotransposon Gag protein that mediates intercellular RNA transfer. cell. 172, 275-288

Latest revision as of 03:11, 12 October 2018


Arc Protein Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for use in E.coli .

Source

The original sequence is derived from the Arc protein found in mouse neural cells. https://www.uniprot.org/uniprot/Q9WV31

References

[1]Pastuzyn, E., Day, C., Keams, R., Kyrke-Smith, M., Taibi, A., McCormick, J., Yoder, N., Belnap, D., Erlendsson, S., Morado, D., Briggs, J., Feschotte, C., and Shepard, J. (2018) The Neuronal Gene Arc encodes a repurposed retrotransposon Gag protein that mediates intercellular RNA transfer. cell. 172, 275-288