Difference between revisions of "Part:BBa K2560133:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part was created by polymerase chain reaction (PCR) on the template <a href="https://parts.igem.org/Part:BBa_K2560057">BBa_K2560057</a> and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly.
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<b> Forward oligo:</b>
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AACGTCTCGCTCGTGCTAAACGGGCAAGGTGTCACC
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<b> Reverse Oligo:</b>
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TTCGTCTCCCTCATGTTAAGATGCGTGATCTGATCCTTC
  
 
===Source===
 
===Source===
  
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===References===
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As Source DNA we used <a href="https://parts.igem.org/Part:BBa_K2560057">BBa_K2560057</a> from our Toolbox.

Latest revision as of 23:41, 17 October 2018


Phytobrick version of KanRes in BBa_K2560002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 146
    Illegal XhoI site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction (PCR) on the template BBa_K2560057 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGTGCTAAACGGGCAAGGTGTCACC

Reverse Oligo: TTCGTCTCCCTCATGTTAAGATGCGTGATCTGATCCTTC

Source

As Source DNA we used BBa_K2560057 from our Toolbox.