Difference between revisions of "Part:BBa K2842720"
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Estrogen receptor protein assembled into BBa_K2842669 (Intein Passenger). | Estrogen receptor protein assembled into BBa_K2842669 (Intein Passenger). | ||
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+ | [[File:T--UCL--IP-NikR and IP-ER gel.png|400px|thumb|left| | ||
+ | <center>'''Figure 1: PCR fragment of functionalised Intein Passenger [https://parts.igem.org/Part:BBa_K2842669 BBa_K2842669]'''</center> | ||
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+ | <p> | ||
+ | (1) NikR with TerL-C intein [https://parts.igem.org/Part:BBa_K2842710 BBa_K2842710]<br /> | ||
+ | (2) ER protein with TerL-C intein [https://parts.igem.org/Part:BBa_K2842720 BBa_K2842720]<br /> | ||
+ | *edited to show relevant bands | ||
+ | </p>''' ]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 16:57, 9 October 2018
Estrogen receptor protein (M421F) with TerL-C intein
Estrogen receptor protein assembled into BBa_K2842669 (Intein Passenger).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1272
Illegal BsaI.rc site found at 28
Illegal SapI site found at 1178
Illegal SapI.rc site found at 419