Difference between revisions of "Part:BBa K2834002"
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<partinfo>BBa_K2834002 short</partinfo> | <partinfo>BBa_K2834002 short</partinfo> | ||
+ | <p align="justify"> | ||
+ | Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).</p><br> | ||
+ | <p> | ||
− | + | Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006 | |
+ | ===References=== | ||
+ | Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254. | ||
+ | </p><br> | ||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
− | = | + | <!-- --> |
− | + | <span class='h3bb'>Sequence and Features</span> | |
+ | <partinfo>BBa_K2834002 SequenceAndFeatures</partinfo> | ||
− | + | <!-- Uncomment this to enable Functional Parameter display | |
− | === | + | ===Functional Parameters=== |
− | + | <partinfo>BBa_K2834002 parameters</partinfo> | |
− | + | <!-- --> | |
− | + | ||
− | + |
Latest revision as of 16:16, 15 October 2018
6x His-Tag
Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).
Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006
References
Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]