Difference between revisions of "Part:BBa K2834002"
 
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<p align="center">hola daniela popó</p>
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__NOTOC__
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<partinfo>BBa_K2834002 short</partinfo>
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<p align="justify">
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Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).</p><br>
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<p>
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Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006
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===References===
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Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.
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</p><br>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2834002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2834002 parameters</partinfo>
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Latest revision as of 16:16, 15 October 2018


6x His-Tag

Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).


Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006

References

Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]