Difference between revisions of "Part:BBa K2619014"

 
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2619014 short</partinfo>
 
<partinfo>BBa_K2619014 short</partinfo>
  
−
Kozak sequence acts as eukaryotic ribosomal binding site for translation initiation.  
+
This part is composed of a concensus Kozak sequence with an nSMase2 protein coding sequence.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2619014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2619014 SequenceAndFeatures</partinfo>
 +
 +
 +
We conducted experiments to test the exosome production boosting ability of our deigned devices (BBa_K2619014—nSMase and BBa_K2619103—Booster) by co-transfection.
 +
[[File:Result2xjtlu.tiff|500|500px|thumb|left|Fig. 1 Exosome production boosting by luciferase assay]]
 +
 +
[[File:Result3xjtlu.png|500|500px|thumb|left|Fig. 2 Exosome production boosting by Pierce BCA protein assay]]
 +
 +
[[File:Result4xjtlu.png|500|500px|thumb|left|Fig. 3 Exosome production boosting by Nanoparticle tracking assay]]
 +
 +
[[File:Result5xjtlu.png|500|500px|thumb|left|Fig. 4 Exosome production boosting by Nanoparticle tracking assay for particles below 100 nm]]
  
  

Latest revision as of 21:43, 17 October 2018


nSMase 2 (neutral sphingomyelinase 2 or Smpd3--sphingomyelin phosphodiesterase 3 ) with Kozak sequen

This part is composed of a concensus Kozak sequence with an nSMase2 protein coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 295
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1617


We conducted experiments to test the exosome production boosting ability of our deigned devices (BBa_K2619014—nSMase and BBa_K2619103—Booster) by co-transfection.

Fig. 1 Exosome production boosting by luciferase assay
Fig. 2 Exosome production boosting by Pierce BCA protein assay
Fig. 3 Exosome production boosting by Nanoparticle tracking assay
Fig. 4 Exosome production boosting by Nanoparticle tracking assay for particles below 100 nm