Difference between revisions of "Part:BBa K2675017"
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===Usage and Biology===
 
===Usage and Biology===
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This RBS was used to drive the expression of sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) under the control of the [[Part:BBa_J23110|BBa_J23110]] promoter in the composite parts [[Part:BBa_K2675052|BBa_K2675052]], [[Part:BBa_K2675062|BBa_K2675062]], ....
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This RBS was used to drive the expression of sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) under the control of the [[Part:BBa_J23110|BBa_J23110]] promoter in the composite parts [[Part:BBa_K2675056|BBa_K2675056]] and [[Part:BBa_K2675066|BBa_K2675066]].
  
 
  Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
 
  Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
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  Translation Initiation Rate (au) : 202865,54
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  Translation Initiation Rate (au) : 18413,48
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  dG_total (kcal/mol) : -11,34
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  dG_total (kcal/mol) : -6,01
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  dG_mRNA_rRNA (kcal/mol) : -20,08
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  dG_mRNA_rRNA (kcal/mol) : -11,31
 
  dG_spacing (kcal/mol) : 0
 
  dG_spacing (kcal/mol) : 0
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  dG_standby (kcal/mol) : 0
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  dG_standby (kcal/mol) : 1,52
 
  dG_start (kcal/mol) : -2,76
 
  dG_start (kcal/mol) : -2,76
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  dG_mRNA (kcal/mol) : -12,28
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  dG_mRNA (kcal/mol) : -7,32
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  Accuracy (warnings) : OK
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  Accuracy (warnings) : NoEQ
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<h2 style="font-weight:800;">REFERENCES</h2>
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<p style="font-size:15px;" class="bibliographie">[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.</p>
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<p style="font-size:15px;" class="bibliographie">[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.</p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 21:05, 15 October 2018


Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the BBa_J23110 promoter in the composite parts BBa_K2675056 and BBa_K2675066. 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	18413,48
dG_total (kcal/mol)	:	-6,01
dG_mRNA_rRNA (kcal/mol)	:	-11,31
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	1,52
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-7,32
Accuracy (warnings)	:	NoEQ

REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]