Difference between revisions of "Part:BBa K2582010"
Frederickk (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2582010 short</partinfo> | <partinfo>BBa_K2582010 short</partinfo> | ||
| − | + | This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) [https://parts.igem.org/Part:BBa_K2582009 BBa_K2582009]. It targets the influenza Polymerase Basic 2 (By Marsh et. al. 2008. J. of Virology) by nucleotide 2247-2268. | |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p>This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of <i>E. coli</i>, it can be expressed by IPTG induction.</p> | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 13:37, 16 October 2018
T7 Promoter-RAP(PB2-2247-2268)-T7 Terminator
This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582009. It targets the influenza Polymerase Basic 2 (By Marsh et. al. 2008. J. of Virology) by nucleotide 2247-2268.
Usage and Biology
This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]