Difference between revisions of "Part:BBa K2582008"
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<partinfo>BBa_K2582008 short</partinfo> | <partinfo>BBa_K2582008 short</partinfo> | ||
| − | + | This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) [https://parts.igem.org/Part:BBa_K2582007 BBa_K2582007]. It targets the influenza Hemagglutinin subtype 7 (Gene accession number: CY235363.1) by nucleotide 474-495. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p>This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of <i>E. coli</i>, it can be expressed by IPTG induction.</p> | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 13:28, 16 October 2018
T7 Promoter-RAP(H7-474-495)-T7 Terminator
This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582007. It targets the influenza Hemagglutinin subtype 7 (Gene accession number: CY235363.1) by nucleotide 474-495.
Usage and Biology
This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 28
- 1000COMPATIBLE WITH RFC[1000]