Difference between revisions of "Part:BBa K2570007"
(5 intermediate revisions by 2 users not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K2570007 short</partinfo> | <partinfo>BBa_K2570007 short</partinfo> | ||
− | This sequence | + | This sequence encodes for atoA, which is the second enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570006 BBa_K2570006], it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570006 BBa_K2570006] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570008 BBa_K2570008], aiming to eliminate unpleasant axilla odors E-3M2H by converting the odorant acids into alcohols. |
+ | |||
+ | [[File:T--FJNU-China--Basic_part-atoA_1.jpg|600px|thumb|center|Fig.1 The structure of atoA.]] | ||
+ | |||
+ | The atoA sequence was obtained from ''E.coli Bw25113'' by PCR | ||
+ | |||
+ | [[File:T--FJNU-China--Basic_part-atoA_2.jpg|600px|thumb|center|Fig.1 PCR result of atoA.]] | ||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 08:47, 16 October 2018
atoA
This sequence encodes for atoA, which is the second enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with BBa_K2570006, it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with BBa_K2570006 and BBa_K2570008, aiming to eliminate unpleasant axilla odors E-3M2H by converting the odorant acids into alcohols.
The atoA sequence was obtained from E.coli Bw25113 by PCR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 180
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]