Difference between revisions of "Part:BBa K2799014"

 
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<partinfo>BBa_K2799014 short</partinfo>
 
<partinfo>BBa_K2799014 short</partinfo>
  
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This BioBrick encompasses the MelA gene for expression in fungi. It has the promoter pTrpC and terminator from the Cauliflower Mosaic Virus (CaMV).
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This BioBrick functions to increase expression of melanin in fungi. It contains <i>melA</i>, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter P<i>trpC</i> derived from <i>Aspergillus nidulans</i>. It's terminator is derived from the Cauliflower Mosaic Virus (tCaMV).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2799014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2799014 SequenceAndFeatures</partinfo>
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<b>Contribution:</b>
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The DTU Biobuilders 2018 demonstrated apparent transformants, which suggests integration of this BioBrick into the genome of <i>A. oryzae</i>. The apparent transformants exhibits darker growth than the surrounding area.
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Latest revision as of 01:01, 18 October 2018


PtrpC- melA - tCaMV

This BioBrick functions to increase expression of melanin in fungi. It contains melA, which encodes a tyrosinase involved in the first step of melanins biosynthesis, under control of the strong constitutive fungal promoter PtrpC derived from Aspergillus nidulans. It's terminator is derived from the Cauliflower Mosaic Virus (tCaMV).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1545
    Illegal BsaI.rc site found at 850


Contribution:

The DTU Biobuilders 2018 demonstrated apparent transformants, which suggests integration of this BioBrick into the genome of A. oryzae. The apparent transformants exhibits darker growth than the surrounding area.