Difference between revisions of "Part:BBa K2623028"

 
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<partinfo>BBa_K2623028 short</partinfo>
 
<partinfo>BBa_K2623028 short</partinfo>
  
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===Summary===
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This part is a composite part constructed by linking <partinfo>BBa_K2623022</partinfo> and <partinfo>BBa_K2623025</partinfo> together. <partinfo>BBa_K2623022</partinfo> can express OmpA inserted with SpyTag (ST) at its N-termini.  <partinfo>BBa_K2623025</partinfo> can express archaeal ribosomal protein protein L7Ae inserted with SpyCatcher (SC) at its N-termini and mRFP1 at its C-termini under the induction of IPTG at 0.5 mM concentration. It's another form of <partinfo>BBa_K2623030</partinfo> in which SpyTag is inserted in the C-termini of OmpA.
  
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Under the induction of IPTG, this part can code SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.
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===Other Information===
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You can visit the Results page: http://2018.igem.org/Team:XMU-China/Results to see the results of comparison of two reporters (<partinfo>BBa_K2623021</partinfo> and <partinfo>BBa_K2623023</partinfo>)
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:56, 14 October 2018


A composite part linked by SpyTag-OmpA and SpyCatcher-L7Ae-mRFP1

Summary

This part is a composite part constructed by linking BBa_K2623022 and BBa_K2623025 together. BBa_K2623022 can express OmpA inserted with SpyTag (ST) at its N-termini. BBa_K2623025 can express archaeal ribosomal protein protein L7Ae inserted with SpyCatcher (SC) at its N-termini and mRFP1 at its C-termini under the induction of IPTG at 0.5 mM concentration. It's another form of BBa_K2623030 in which SpyTag is inserted in the C-termini of OmpA.

Other Information

You can visit the Results page: http://2018.igem.org/Team:XMU-China/Results to see the results of comparison of two reporters (BBa_K2623021 and BBa_K2623023) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1965
    Illegal NheI site found at 1988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 3122
    Illegal AgeI site found at 3234
  • 1000
    COMPATIBLE WITH RFC[1000]