Difference between revisions of "Part:BBa K2549004:Design"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2549004 short</partinfo>
 
<partinfo>BBa_K2549004 short</partinfo>
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<partinfo>BBa_K2549004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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LaG17 is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP[1].
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LaG17<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref> is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP. This nanobody recognizes our surEGFP ([[Part:BBa_K2446051]]) expressing cells.
  
 
===Source===
 
===Source===
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from IDT(gBlock), codon optimized for human
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From IDT (gBlock), codon optimized for human
  
 
===References===
 
===References===
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[1]A robust pipeline for rapid production of versatile nanobody repertoires.
 
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Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP.
 
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Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170
 

Latest revision as of 13:13, 12 October 2018


anti-GFP (LaG17)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


Design Notes

LaG17[1] is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170