Difference between revisions of "Part:BBa K2680267:Design"

 
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===Design Notes===
 
===Design Notes===
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To do
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Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
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===Source===
 
===Source===
  
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To do
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Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.
  
 
===References===
 
===References===

Latest revision as of 16:10, 16 October 2018


3G GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 783


Design Notes

Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.

References