Difference between revisions of "Part:BBa K2549002:Design"

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__NOTOC__
 
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<partinfo>BBa_K2549002 short</partinfo>
 
<partinfo>BBa_K2549002 short</partinfo>
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<partinfo>BBa_K2549002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549002 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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When fused with LaG16 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1].
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Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. LaG2 fuses two copies of LaG16 ([[Part:BBa_K2549002]]) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>.
  
 
===Source===
 
===Source===
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From IDT (gBlock), codon optimized for human
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IDT(gBlock)
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===References===
 
===References===
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[1]A robust pipeline for rapid production of versatile nanobody repertoires.
 
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Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP.
 
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Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170
 

Latest revision as of 13:12, 12 October 2018


anti-GFP (LaG2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anti-GFP (LaG2) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. LaG2 fuses two copies of LaG16 (Part:BBa_K2549002) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity antibody against GFP[1].

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170