Difference between revisions of "Part:BBa K2685007"

 
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2685007 short</partinfo>
 
<partinfo>BBa_K2685007 short</partinfo>
  
−
tetracycline detecting parts
+
<p>This part is our part of the test to detect tetracycline in the environment. Because <i>E. coli</i> is not tolerant to tetracycline, we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration of tetracycline. The ABS value of <i>E. coli</i> was determined by comparing with that of <i>E.coli</i>. The results showed that the highest concentration of tetracycline was 10μg/ml in<i> E.coli</i> and 110μg /ml in <i>E.coli</i>.</p>
 +
  <div class="at-user__avatar" align="center">
 +
"https://static.igem.org/mediawiki/parts/e/e6/T--TUST_China--part_document_072.png"
 +
                    </div>
 +
<p>The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.</p>
 +
<div class="at-user__avatar" align="center">
 +
"https://static.igem.org/mediawiki/parts/7/7c/T--TUST_China--MIC.png"
 +
                    </div>
 +
<p>Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance is more stable and the detection accuracy is higher.</p>
 +
<div class="at-user__avatar" align="center">
 +
"https://static.igem.org/mediawiki/parts/c/c9/T--TUST_China--4.png"
 +
                    </div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:49, 17 October 2018


Detecting parts

This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline, we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.

"T--TUST_China--part_document_072.png"

The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.

"T--TUST_China--MIC.png"

Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance is more stable and the detection accuracy is higher.

"T--TUST_China--4.png"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1768
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1622
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1068
    Illegal NgoMIV site found at 1228
    Illegal NgoMIV site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69