Difference between revisions of "Part:BBa K2637028:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | TDH3 Promoter + BD + KaiC + ADH1 Terminator. And KaiC has been optimized in yeast. | |
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===Source=== | ===Source=== | ||
| − | + | TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942. | |
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===References=== | ===References=== | ||
Latest revision as of 06:23, 14 October 2018
BD-KaiC cassette with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
Illegal BglII site found at 1782
Illegal BglII site found at 2289
Illegal XhoI site found at 1291 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1210
Design Notes
TDH3 Promoter + BD + KaiC + ADH1 Terminator. And KaiC has been optimized in yeast.
Source
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942.