Difference between revisions of "Part:BBa K2558202"

 
 
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<partinfo>BBa_K2558202 short</partinfo>
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This part contains constantly expressed lacI, and plac-controlled sfGFP as our reporter. We aim to use this construct to test the function of lacI-regulated, lambda pL hybrid promoter by treating the bacteria with different dosages of IPTG and measuring the strength of GFP expression. 
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===Usage and Biology===
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===Sequence and Features===
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<partinfo>BBa_K2558202 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2558202 parameters</partinfo>
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Latest revision as of 14:34, 17 October 2018


lacI-regulated, lambda pL hybrid promotor test device

This part contains constantly expressed lacI, and plac-controlled sfGFP as our reporter. We aim to use this construct to test the function of lacI-regulated, lambda pL hybrid promoter by treating the bacteria with different dosages of IPTG and measuring the strength of GFP expression. 

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
    Illegal NheI site found at 176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]