Difference between revisions of "Part:BBa K2675015"

Latest revision as of 21:05, 15 October 2018

Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX short promoter (BBa_K2675021) or its derivative (BBa_K2675022) in the composite parts BBa_K2675051, BBa_K2675052, BBa_K2675058, BBa_K2675059, BBa_K2675061 & BBa_K2675062.

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	202865,54
dG_total (kcal/mol)	:	-11,34
dG_mRNA_rRNA (kcal/mol)	:	-20,08
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-12,28
Accuracy (warnings)	:	OK

REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2675015 short</partinfo>
-Synthetic RBS designed for sfGFP
+This part is a synthetic RBS designed for sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) using the [https://salislab.net/software/forward Salis Lab RBS calculator v2.0] [1, 2].
-used with pAimX-Phi3T short promoter
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This RBS was used to drive the expression of sfGFP ([[Part:BBa_K2675005|BBa_K2675005]]) and sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) under the control of the pAimX short promoter ([[Part:BBa_K2675021|BBa_K2675021]]) or its derivative ([[Part:BBa_K2675022|BBa_K2675022]]) in the composite parts [[Part:BBa_K2675051|BBa_K2675051]], [[Part:BBa_K2675052|BBa_K2675052]], [[Part:BBa_K2675058|BBa_K2675058]], [[Part:BBa_K2675059|BBa_K2675059]],  [[Part:BBa_K2675061|BBa_K2675061]] & [[Part:BBa_K2675062|BBa_K2675062]].
+ Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
+ Translation Initiation Rate (au)	:	202865,54
+ dG_total (kcal/mol)	:	-11,34
+ dG_mRNA_rRNA (kcal/mol)	:	-20,08
+ dG_spacing (kcal/mol)	:	0
+ dG_standby (kcal/mol)	:	0
+ dG_start (kcal/mol)	:	-2,76
+ dG_mRNA (kcal/mol)	:	-12,28
+ Accuracy (warnings)	:	OK
+<h2 style="font-weight:800;">REFERENCES</h2>
+<p style="font-size:15px;" class="bibliographie">[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.</p>
+<p style="font-size:15px;" class="bibliographie">[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.</p>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>