Difference between revisions of "Part:BBa K2540010"

 
 
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<partinfo>BBa_K2540010 short</partinfo>
 
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This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 29572) and is originally found in Eubacterium rectale.  
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This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 29572) and is originally found in <i>Eubacterium rectale</i>.  
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<img src="https://static.igem.org/mediawiki/2018/a/ad/T--Rice--HW004.png" width = 70%>
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<p><b>Table 1</b>: Relative transcription and translation levels for the broad host range element (strength 10).</p>
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<p> The part was characterized in four <i>E. coli</i> strains, <i>S. oneidensis </i>, and <i>P. putida </i> using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB at 30C for 24 hours.
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<img src="https://static.igem.org/mediawiki/2018/d/d0/T--Rice--HW004_data.png" width = 50%>
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<p><b>Figure 1</b>: Fluorescence levels of mKate2 under the control of the broad host range element (strength 10).</p>
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2540010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2540001 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2540010 parameters</partinfo>
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<partinfo>BBa_K2540001 parameters</partinfo>
 
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===References===
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Johns, N. I. <i>et al.</i>(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.

Latest revision as of 19:14, 10 October 2018


Broad host range regulatory element (strength 10)

This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 29572) and is originally found in Eubacterium rectale.


Table 1: Relative transcription and translation levels for the broad host range element (strength 10).


The part was characterized in four E. coli strains, S. oneidensis , and P. putida using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB at 30C for 24 hours.

Figure 1: Fluorescence levels of mKate2 under the control of the broad host range element (strength 10).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 183



References

Johns, N. I. et al.(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.