Difference between revisions of "Part:BBa K2541002:Design"

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===Design Notes===
 
===Design Notes===
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When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.
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The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.
  
 
===Source===
 
===Source===
  
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Our construction device includes a promoter J23101 and chromoprotein tspurple, which can be replaced by the BbsI restriction endonuclease, cjblue that can be replaced by the BsaI restriction endonuclease, conserved sequence and reporter protein sfGFP. Such a composition can be realized that when both the replacement part I and the replacement part II are not replaced, the colony exhibits the color of the tspurple, and when the replacement part I is replaced with another promoter by using BbsI, the colony appears blue, and the unsuccessful colony appears Purple. When BsaI was used to replace Part II, the colony showed the original milky white color of the bacteria, and the blue color colony is unsuccess.
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This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.
  
 
===References===
 
===References===
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[1]Neupert J, Karcher D, Bock R. Design of simple synthetic RNA thermometers for temperature-controlled gene expression, in Escherichia coli.[J]. Nature Protocols, 2008, 4(9):1262-73.
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[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.
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[3]Neupert J, Bock R. Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria. Nature Protocols, 2009, 4(9):1262-73.
 +
 +
[4]Sen S, Apurva D, Satija R, et al. Design of a Toolbox of RNA Thermometers[J]. Acs Synthetic Biology, 2017, 6(8).

Latest revision as of 09:45, 13 October 2018


Heat-inducible RNA-based thermosensor-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.

Source

This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.

References

[1]Neupert J, Karcher D, Bock R. Design of simple synthetic RNA thermometers for temperature-controlled gene expression, in Escherichia coli.[J]. Nature Protocols, 2008, 4(9):1262-73.

[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.

[3]Neupert J, Bock R. Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria. Nature Protocols, 2009, 4(9):1262-73.

[4]Sen S, Apurva D, Satija R, et al. Design of a Toolbox of RNA Thermometers[J]. Acs Synthetic Biology, 2017, 6(8).