Difference between revisions of "Part:BBa K2889000"

Latest revision as of 02:56, 8 October 2018

pSB1C3-IL7-AS-S2

IL7-AS [located anti-sense to interleukin-7 (Il7) gene; Accession number: NM_000880.3] is a newly discovered lncRNA that can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6). IL6 is emerging as a major regulator of renal cell cancer. However, roles of IL7-AS in renal cell cancer and the related molecular mechanisms remain largely undetermined.We cloned the truncated sequences of IL7-AS (IL7-AS-S2 contain 411bp) into pSB1C3 for submitting. And we also cloned IL7-AS-S2 into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S2 contain key structural domains.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 198
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2889000 short</partinfo>
-We cloned the full length of IL7-AS, two truncated sequences of IL7-AS (IL7-AS-S1 and IL7-AS-S2) into PCDNA3.1 and transfected these plasmids to 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS promoted cell migration of 786-O cells. However IL7-AS-S1 and IL7-AS-S2 did not influence the migration of 786-O cells, suggesting IL7-AS-S1 and IL7-AS-S2 lack of key structural domains.
+IL7-AS [located anti-sense to interleukin-7 (Il7) gene; Accession number: NM_000880.3] is a newly discovered lncRNA that can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6).  IL6 is emerging as a major regulator of renal cell cancer. However, roles of IL7-AS in renal cell cancer and the related molecular mechanisms remain largely undetermined.We cloned the truncated sequences of IL7-AS (IL7-AS-S2 contain 411bp) into pSB1C3 for submitting. And we also cloned IL7-AS-S2 into PCDNA3.1 and transfected these plasmids into 786-O cells. Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells. These results suggested that IL7-AS-S2 contain key structural domains.
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