Difference between revisions of "Part:BBa K2570007"
 
(6 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2570007 short</partinfo>
 
<partinfo>BBa_K2570007 short</partinfo>
  
−
This sequence codes for atoA, which is the first enzyme responsible for degrading unpleasant axilla odors E-3M2H( E-3-methyl-3-hexenoic acid).Together with BBa_K2570006, it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with BBa_K2570006 and BBa_K2570008, aiming to eliminate unpleasant axilla odors E-3M2H by converting acids into alcohols.
+
This sequence encodes for atoA, which is the second enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570006 BBa_K2570006], it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570006 BBa_K2570006] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570008 BBa_K2570008], aiming to eliminate unpleasant axilla odors E-3M2H by converting the odorant acids into alcohols.
−
<img href="https://static.igem.org/mediawiki/2017/9/9c/T--TUDelft--homepage_projectscheme.png" style="width:10% !important;">
+
 
 +
[[File:T--FJNU-China--Basic_part-atoA_1.jpg|600px|thumb|center|Fig.1 The structure of atoA.]]
 +
 
 +
The atoA sequence was obtained from ''E.coli Bw25113'' by PCR
 +
 
 +
[[File:T--FJNU-China--Basic_part-atoA_2.jpg|600px|thumb|center|Fig.1 PCR result of atoA.]]
 +
 
 +
 
 +
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 08:47, 16 October 2018


atoA

This sequence encodes for atoA, which is the second enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).Together with BBa_K2570006, it can add coA to E-3M2H for fatty acid activation and its subsequent degradation. These works are in conjunctions with BBa_K2570006 and BBa_K2570008, aiming to eliminate unpleasant axilla odors E-3M2H by converting the odorant acids into alcohols.

Fig.1 The structure of atoA.

The atoA sequence was obtained from E.coli Bw25113 by PCR

Fig.1 PCR result of atoA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]