Difference between revisions of "Part:BBa K2623024"
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<partinfo>BBa_K2623024 short</partinfo> | <partinfo>BBa_K2623024 short</partinfo> | ||
| − | This part | + | This part is the non-GFP form of <partinfo>BBa_K2623023</partinfo>. It's also another form of <partinfo>BBa_K2623022</partinfo> in which SpyTag is inserted to the C-termini of OmpA and is designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We insert SpyTag to its C-termini and SpyCather to L7Ae (<partinfo>BBa_K2623026</partinfo>) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system. |
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Latest revision as of 03:52, 14 October 2018
A non-GFP form of the BBa_K2623023
This part is the non-GFP form of BBa_K2623023. It's also another form of BBa_K2623022 in which SpyTag is inserted to the C-termini of OmpA and is designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We insert SpyTag to its C-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
- 1000COMPATIBLE WITH RFC[1000]