Difference between revisions of "Part:BBa K2623023"

 
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<partinfo>BBa_K2623023 short</partinfo>
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===Summary===
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It’s another form of <partinfo>BBa_K2623021</partinfo> designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we insert SpyTag to the C-termini of OmpA protein(instead of the N-termini in <partinfo>BBa_K2623021</partinfo>). As mentioned in <partinfo>BBa_K2623021</partinfo>, OmpA is constructed with SpyTag in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the <partinfo>BBa_K2623021</partinfo> and this part. Then the better one will be uesd for downstream analysis.
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===Other Information===
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You can visit the Results page: http://2018.igem.org/Team:XMU-China/Results to see the results of comparison of two reporters (<partinfo>BBa_K2623021</partinfo> and <partinfo>BBa_K2623023</partinfo>)
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Sequence and Features</span>
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<partinfo>BBa_K2623022 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2623022 parameters</partinfo>
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Latest revision as of 03:50, 14 October 2018


Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini

Summary

It’s another form of BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we insert SpyTag to the C-termini of OmpA protein(instead of the N-termini in BBa_K2623021). As mentioned in BBa_K2623021, OmpA is constructed with SpyTag in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the BBa_K2623021 and this part. Then the better one will be uesd for downstream analysis.

Other Information

You can visit the Results page: http://2018.igem.org/Team:XMU-China/Results to see the results of comparison of two reporters (BBa_K2623021 and BBa_K2623023)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]