Difference between revisions of "Part:BBa K2753055"

 
 
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<partinfo>BBa_K2753055 short</partinfo>
 
<partinfo>BBa_K2753055 short</partinfo>
  
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One mutation of pTALE library
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The promoter UP119 is a modified variant of J23119 consensus promoter ([[Part:BBa_J23119]]) by adding an up-element. This alteration makes UP119 stronger than the original J23119.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<html>
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<center>
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<Figure>
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<img width="70%" src="https://static.igem.org/mediawiki/2018/9/9e/T--GreatBay_China--119_design.png">
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</figure>
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</center>
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</html>
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<br/>
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<p>By changing the sequence of UP119 to create a binding site for TALE2, we obtained another J23119 variant and has made it the core promoter sequence of a member from the TALE stabilised promoter family, pTALE2 sp6.</p>
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===Characterisation in comparison to J23119===
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<p>For comparing the strength of two J23119 variants with J23119, we cloned them with a sfGFP onto three plasmids of distinctive copy numbers.</p>
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<ul>
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<li>pUC20: ~500</li>
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<li>pR6K: ~15</li>
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<li>pSC101: ~1</li>
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</ul>
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<br>
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<p>The strength of them are characterised by measuring the fluorescence using a Flow Cytometry.</p>
  
 
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Latest revision as of 15:17, 13 October 2018


UPJ23119

The promoter UP119 is a modified variant of J23119 consensus promoter (Part:BBa_J23119) by adding an up-element. This alteration makes UP119 stronger than the original J23119.


For comparing the strength of two J23119 variants with J23119, we cloned them with a sfGFP onto three plasmids of distinctive copy numbers.

  • pUC20: ~500
  • pR6K: ~15
  • pSC101: ~1


The strength of them are characterised by measuring the fluorescence using a Flow Cytometry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]