Difference between revisions of "Part:BBa K2669003:Design"
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===Design Notes===
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===Source===
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The composite part is equipped with primer binding sites for VF2 and VR and contains Biobrick scar sequence.
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The sequence was codon optimized with the codon optimization tool provided by IDT.
  
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Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT)
  
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===Source===
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constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).
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RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014) primer binfing sites VF2 and VR (BBa_G00100), (BBa_G00102).  
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===References===
 
===References===

Latest revision as of 03:15, 18 October 2018


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequence was codon optimized with the codon optimization tool provided by IDT.

Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT)

constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).

References