Difference between revisions of "Part:BBa K2865010"

 
 
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<partinfo>BBa_K2865010 short</partinfo>
 
<partinfo>BBa_K2865010 short</partinfo>
  
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<p class="MsoNormal"><span lang="EN-US">This is a mammalian eGFP generator which was used to verify the CMV promoter can actually promote the protein AR185 expression and was used as a control of the parts BNP-AR185-Poly(A) (BBa_K2865009). This part contains the CMV promoter sequence followed by AR185-T2A-EGFP (BBa_K2865001), and SV40 polyA terminator (BBa_K2865004). We have clearly description about AR185 and Poly(A) gene.</span></p>
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===Experiment===
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===Usage and Biology===
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<p class="MsoNormal"><span lang="EN-US">We transfected H9C2 cell with this part by liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. Because of the low cell division rate and cell specificity, we have to admit that the transfection efficiency is low. But in the cell experiment, we have got credible evidence to prove the hypothesis. </span></p>
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<p class="MsoNormal" style="text-indent: 0cm;"><span lang="EN-US">&nbsp;</span></p>[[File:T--SMMU-China--ic185 2.png|400px|thumb|center|Figure 1. The fluorescence expression of CMV-AR185-Poly(A) 48 hours later ]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 04:26, 17 October 2018


CMV-AR185-Poly(A)

This is a mammalian eGFP generator which was used to verify the CMV promoter can actually promote the protein AR185 expression and was used as a control of the parts BNP-AR185-Poly(A) (BBa_K2865009). This part contains the CMV promoter sequence followed by AR185-T2A-EGFP (BBa_K2865001), and SV40 polyA terminator (BBa_K2865004). We have clearly description about AR185 and Poly(A) gene.

Experiment

We transfected H9C2 cell with this part by liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. Because of the low cell division rate and cell specificity, we have to admit that the transfection efficiency is low. But in the cell experiment, we have got credible evidence to prove the hypothesis.

 

Figure 1. The fluorescence expression of CMV-AR185-Poly(A) 48 hours later

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 817
    Illegal NgoMIV site found at 964
    Illegal NgoMIV site found at 1899
  • 1000
    COMPATIBLE WITH RFC[1000]