Difference between revisions of "Part:BBa K2558201"
 
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Part K2558201 is a generator of dCas9, which is the non-enzymatic mutant of Cas9, an DNase. dCas9 remains the ability bind with specific gRNA and the DNA to which the gRNA is complemental, but does not cut  DNA helixes. Therefore dCas9-gRNA complex is able to inhibit expression without damaging DNA. As dCas9 is detrimental to the bacteria’s growth, we put it after promoter J23114 with B0030 which makes sure dCas9 will be expressed at a constant level without affecting the bacteria.
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__NOTOC__
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<partinfo>BBa_K2558201 short</partinfo>
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Part K2558201 is a generator of dCas9, which is a mutant of Cas9 without the DNase activity. dCas9 preserves the ability to bind specific gRNA and then gRNA’s complementary DNA strand. Therefore dCas9/gRNA complex is able to inhibit expression without damaging DNA. As dCas9 is detrimental to the growth of bacteria, we put it after promoter J23114 with B0030 to make sure that dCas9 will be expressed at a constant level with minimal side effect on bacterial growth.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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===Sequence and Features===
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<partinfo>BBa_K2558201 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2558201 parameters</partinfo>
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Latest revision as of 14:31, 17 October 2018


dCas9 generator with Anderson weak promotor

Part K2558201 is a generator of dCas9, which is a mutant of Cas9 without the DNase activity. dCas9 preserves the ability to bind specific gRNA and then gRNA’s complementary DNA strand. Therefore dCas9/gRNA complex is able to inhibit expression without damaging DNA. As dCas9 is detrimental to the growth of bacteria, we put it after promoter J23114 with B0030 to make sure that dCas9 will be expressed at a constant level with minimal side effect on bacterial growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]