Difference between revisions of "Part:BBa K2835000:Design"

Latest revision as of 15:47, 1 October 2018

Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part is an improved version of BBa_I13502. To make the BioBrick compatible to assembly standard RFC25, two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.

Source

The RFP gene is originally derived from Discosoma striata (coral). The mRFP1 gene is originally derived from a monomeric derived version of RFP, GenBank: AF506027.1

@@ Line 9: / Line 9: @@
 This part is an improved version of <partinfo>BBa_I13502</partinfo>.
-To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
+To make the BioBrick compatible to assembly standard RFC25, two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
 ===Source===
-Part is originally derived from GenBank: AF506027.1 <br>
+The RFP gene is originally derived from ''Discosoma striata'' (coral). The mRFP1 gene is originally derived from a monomeric derived version of RFP, GenBank: AF506027.1
-https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 <br>
-from Discosoma striata (coral)
 ===References===